PrimePCR™ SYBR® Green Assay: Plk4, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: Plk4, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Plk4, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCID0016093
Assay Design:		Intron-spanning
Chromosome Location:		3:40815060-40816411question
Amplicon Length:		96
Splice Variants Targeted:		ENSMUST00000026858 ENSMUST00000168287 ENSMUST00000167556

Gene Information

This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to the nucleolus during G2 to centrosomes during G2/M and to the cleavage furrow during cytokinesis. It is required for progression through mitosis cell survival and embryonic development. The mouse genome contains a pseudogene similar to this gene. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq Nov 2011]

Gene Symbol:		Plk4
Gene Name:		Polo-like kinase 4 (Drosophila)
Aliases:		1700028H20, AI385771, Sak, Stk18
RefSeq:		NC_000069.6 NT_039229.7
Ensembl:		ENSMUSG00000025758
Entrez:		20873
Chromosome Mapping:		3 B\|3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999600
y-intercept	36.270000
Efficiency	94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.