PrimePCR™ SYBR® Green Assay: Rest, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0032738

List Price:    $174.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0018707
Assay Design:   Intron-spanning
Chromosome Location:   5:77268743-77275299question
Amplicon Length:   117
Splice Variants Targeted:   ENSMUST00000080359 ENSMUST00000113449

Gene Information

Description Not Available

Gene Symbol:   Rest
Gene Name:   RE1-silencing transcription factor
Aliases:   2610008J04Rik, AA407358, NRSF
RefSeq:   NC_000071.6 NT_109320.5
Ensembl:   ENSMUSG00000029249
Entrez:   19712
Chromosome Mapping:   5 C3.3|5

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.370000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download