PrimePCR™ SYBR® Green Assay: Dnmt3b, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: Dnmt3b, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Dnmt3b, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCID0019749
Assay Design:		Intron-spanning
Chromosome Location:		2:153666055-153667546question
Amplicon Length:		60
Splice Variants Targeted:		ENSMUST00000109773 ENSMUST00000072997 ENSMUST00000103151 ENSMUST00000081628 ENSMUST00000109774 ENSMUST00000103150 ENSMUST00000109772 ENSMUST00000088976 ENSMUST00000109771 ENSMUST00000056495

Gene Information

This is one of two related genes encoding de novo DNA methyltransferases which are responsible for the establishment of DNA methylation patterns in embryos. Loss of function of this gene results in severe developmental defects and loss of viability. Mutation of the related gene in humans causes immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. There is a pseudogene for this gene located adjacent to this gene in the same region of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq Nov 2012]

Gene Symbol:		Dnmt3b
Gene Name:		DNA methyltransferase 3B
Aliases:		Not Available
RefSeq:		NC_000068.7 NT_039207.8
Ensembl:		ENSMUSG00000027478
Entrez:		13436
Chromosome Mapping:		2\|2 A2-A3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999000
y-intercept	34.990000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.