PrimePCR™ SYBR® Green Assay: Uchl3, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: Uchl3, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Uchl3, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCID0020693
Assay Design:		Intron-spanning
Chromosome Location:		14:101654021-101665860question
Amplicon Length:		167
Splice Variants Targeted:		ENSMUST00000006444 ENSMUST00000039229 ENSMUST00000003876 ENSMUST00000115765 ENSMUST00000120267 ENSMUST00000122116 ENSMUST00000023061 ENSMUST00000064473 ENSMUST00000137551 ENSMUST00000069817 ENSMUST00000099981 ENSMUST00000134720 ENSMUST00000148747 ENSMUST00000111882 ENSMUST00000111846 ENSMUST00000034203 ENSMUST00000174398 ENSMUST00000111404 ENSMUST00000111254 ENSMUST00000020315 ENSMUST00000053734 ENSMUST00000039011 ENSMUST00000057610 ENSMUST00000113200 ENSMUST00000028132 ENSMUST00000078588 ENSMUS

Gene Information

Description Not Available

Gene Symbol:		Uchl3
Gene Name:		Ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase)
Aliases:		Not Available
RefSeq:		NC_000080.6 NT_039606.8
Ensembl:		ENSMUSG00000022111
Entrez:		50933
Chromosome Mapping:		14 E2.3\|14 48.0 cM

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	35.390000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.