PrimePCR™ SYBR® Green Assay: Ifrd2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Ifrd2, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Ifrd2, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020697
Assay Design:   Intron-spanning
Chromosome Location:   9:107587767-107589947question
Amplicon Length:   195
Splice Variants Targeted:   ENSMUST00000050770 ENSMUST00000168818 ENSMUST00000166890 ENSMUST00000164752 ENSMUST00000166102 ENSMUST00000164037 ENSMUST00000045665 ENSMUST00000168381 ENSMUST00000086444 ENSMUST00000064795 ENSMUST00000165033 ENSMUST00000025483 ENSMUST00000003850 ENSMUST00000043983 ENSMUST00000038448 ENSMUST00000010192 ENSMUST00000039987 ENSMUST00000159062 ENSMUST00000161078 ENSMUST00000161546 ENSMUST00000069098 ENSMUST00000118607 ENSMUST00000030948 ENSMUST00000168552 ENSMUST00000053407 ENSMUST00000113182 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   Ifrd2
Gene Name:   Interferon-related developmental regulator 2
Aliases:   1810034A24Rik, AA470234, AI838527, SKMc15
RefSeq:   NC_000075.6 NT_039477.8
Ensembl:   ENSMUSG00000010048
Entrez:   15983
Chromosome Mapping:   9 F1|9 60.21 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.040000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download