PrimePCR™ SYBR® Green Assay: Il25, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best efficiency


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PrimePCR™ PreAmp for SYBR® Green Assay: Il25, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Il25, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCID0020756
Assay Design:		Intron-spanning
Chromosome Location:		14:54933084-54935130question
Amplicon Length:		139
Splice Variants Targeted:		ENSMUST00000054651 ENSMUST00000002121 ENSMUST00000098644 ENSMUST00000049681 ENSMUST00000037863 ENSMUST00000000910 ENSMUST00000030025 ENSMUST00000153369 ENSMUST00000164273 ENSMUST00000065977 ENSMUST00000102736 ENSMUST00000106644 ENSMUST00000009256 ENSMUST00000074829 ENSMUST00000098362 ENSMUST00000081381 ENSMUST00000113111 ENSMUST00000102800 ENSMUST00000113099 ENSMUST00000028224 ENSMUST00000031224 ENSMUST00000060311

Gene Information

Description Not Available

Gene Symbol:		Il25
Gene Name:		Interleukin 25
Aliases:		IL-17e, Il17e
RefSeq:		NC_000080.6 NT_039606.8
Ensembl:		ENSMUSG00000040770
Entrez:		140806
Chromosome Mapping:		14 C3\|14

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	35.250000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.