PrimePCR™ SYBR® Green Assay: Htr5a, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: Htr5a, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Htr5a, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCID0020772
Assay Design:		Intron-spanning
Chromosome Location:		5:27843122-27850908question
Amplicon Length:		193
Splice Variants Targeted:		ENSMUST00000091002 ENSMUST00000107689 ENSMUST00000001179 ENSMUST00000010804 ENSMUST00000055911 ENSMUST00000033173 ENSMUST00000106483 ENSMUST00000087050 ENSMUST00000179893 ENSMUST00000032813 ENSMUST00000080145 ENSMUST00000109694 ENSMUST00000043141 ENSMUST00000107757 ENSMUST00000107756 ENSMUST00000030021 ENSMUST00000007977 ENSMUST00000020927 ENSMUST00000072836 ENSMUST00000118687 ENSMUST00000027472 ENSMUST00000105511 ENSMUST00000045529 ENSMUST00000035208 ENSMUST00000132517 ENSMUST00000171637 ENSMUS

Gene Information

Description Not Available

Gene Symbol:		Htr5a
Gene Name:		5-hydroxytryptamine (serotonin) receptor 5A
Aliases:		Htr5
RefSeq:		NC_000071.6 NT_165760.3
Ensembl:		ENSMUSG00000039106
Entrez:		15563
Chromosome Mapping:		5 A3-B\|5 15.0 cM

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.998800
y-intercept	35.560000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.