PrimePCR™ SYBR® Green Assay: Gapt, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: Gapt, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Gapt, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020884
Assay Design:   Intron-spanning
Chromosome Location:   13:110354443-110357163question
Amplicon Length:   188
Splice Variants Targeted:   ENSMUST00000168579 ENSMUST00000113183 ENSMUST00000118821 ENSMUST00000113176 ENSMUST00000068340 ENSMUST00000002081 ENSMUST00000105971 ENSMUST00000105973 ENSMUST00000096363 ENSMUST00000122232 ENSMUST00000120753 ENSMUST00000107362 ENSMUST00000058806

Gene Information

Description Not Available

Gene Symbol:   Gapt
Gene Name:   Grb2-binding adaptor, transmembrane
Aliases:   9830130M13Rik
RefSeq:   NC_000079.6 NT_039590.8
Ensembl:   ENSMUSG00000046006
Entrez:   238875
Chromosome Mapping:   13 D2.2|13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.390000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download