PrimePCR™ SYBR® Green Assay: Slc32a1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: Slc32a1, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Slc32a1, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCID0020904
Assay Design:		Intron-spanning
Chromosome Location:		2:158611588-158613937question
Amplicon Length:		135
Splice Variants Targeted:		ENSMUST00000084843 ENSMUST00000124189 ENSMUST00000094129 ENSMUST00000106943 ENSMUST00000094348 ENSMUST00000103225 ENSMUST00000179332 ENSMUST00000035375 ENSMUST00000105854 ENSMUST00000001963 ENSMUST00000132280 ENSMUST00000172171 ENSMUST00000166825 ENSMUST00000001826 ENSMUST00000045738 ENSMUST00000119878 ENSMUST00000023614

Gene Information

Description Not Available

Gene Symbol:		Slc32a1
Gene Name:		Solute carrier family 32 (GABA vesicular transporter), member 1
Aliases:		R75019, VGAT, Viaat
RefSeq:		NC_000068.7 NT_039207.8
Ensembl:		ENSMUSG00000037771
Entrez:		22348
Chromosome Mapping:		2\|2 H1-3

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.998300
y-intercept	36.120000
Efficiency	93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.