PrimePCR™ SYBR® Green Assay: Klk1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: Klk1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Klk1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020954
Assay Design:   Intron-spanning
Chromosome Location:   7:44225437-44227655question
Amplicon Length:   193
Splice Variants Targeted:   ENSMUST00000042701 ENSMUST00000102524 ENSMUST00000159756 ENSMUST00000025908 ENSMUST00000030469 ENSMUST00000015346 ENSMUST00000049908 ENSMUST00000074359 ENSMUST00000075162 ENSMUST00000105321 ENSMUST00000021411 ENSMUST00000107498 ENSMUST00000090942 ENSMUST00000081695 ENSMUST00000126245 ENSMUST00000175741 ENSMUST00000108146 ENSMUST00000176518 ENSMUST00000064762 ENSMUST00000097774 ENSMUST00000105670 ENSMUST00000105668 ENSMUST00000108303 ENSMUST00000081933 ENSMUST00000071076 ENSMUST00000172951

Gene Information

Description Not Available

Gene Symbol:   Klk1
Gene Name:   Kallikrein 1
Aliases:   0610007D04Rik, Kal, Klk1b6, Klk6, mGk-6
RefSeq:   NC_000073.6 NT_187035.1
Ensembl:   ENSMUSG00000063903
Entrez:   16612
Chromosome Mapping:   7 B4|7 23.0 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.190000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download