PrimePCR™ SYBR® Green Assay: Marcks, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: Marcks, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Marcks, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCID0020960
Assay Design:		Intron-spanning
Chromosome Location:		10:37136856-37138580question
Amplicon Length:		197
Splice Variants Targeted:		ENSMUST00000086210 ENSMUST00000172743 ENSMUST00000167771 ENSMUST00000098006 ENSMUST00000084474 ENSMUST00000052107 ENSMUST00000091851 ENSMUST00000109896 ENSMUST00000030014 ENSMUST00000027455 ENSMUST00000041537 ENSMUST00000021028 ENSMUST00000166232 ENSMUST00000092995 ENSMUST00000030826 ENSMUST00000170580 ENSMUST00000092584 ENSMUST00000006559 ENSMUST00000130451 ENSMUST00000052590 ENSMUST00000064709 ENSMUST00000119254 ENSMUST00000098614 ENSMUST00000137724 ENSMUST00000030879 ENSMUST00000161908 ENSMUS

Gene Information

Description Not Available

Gene Symbol:		Marcks
Gene Name:		Myristoylated alanine rich protein kinase C substrate
Aliases:		Macs, PKCSL
RefSeq:		NC_000076.6 NT_039492.8
Ensembl:		ENSMUSG00000069662
Entrez:		17118
Chromosome Mapping:		10 B1\|10 22.0 cM

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	35.480000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.