PrimePCR™ SYBR® Green Assay: CAAA01154301.1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: CAAA01154301.1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CAAA01154301.1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0020984
Assay Design:   Intron-spanning
Chromosome Location:   9:124422797-124424000question
Amplicon Length:   91
Splice Variants Targeted:   ENSMUST00000031017 ENSMUST00000115789 ENSMUST00000180270 ENSMUST00000101506 ENSMUST00000156550 ENSMUST00000033004 ENSMUST00000069623 ENSMUST00000097820 ENSMUST00000105799 ENSMUST00000105798 ENSMUST00000105797 ENSMUST00000039204 ENSMUST00000166758 ENSMUST00000026585 ENSMUST00000117634 ENSMUST00000020399 ENSMUST00000108809 ENSMUST00000162538 ENSMUST00000096397 ENSMUST00000105321 ENSMUST00000064454 ENSMUST00000094427 ENSMUST00000107498 ENSMUST00000090942 ENSMUST00000081695 ENSMUST00000024049 ENSMUS

Gene Information

Description Not Available

Gene Symbol:   CAAA01154301.1
Gene Name:   Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha isoform 1
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSMUSG00000093803
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 38.650000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download