PrimePCR™ SYBR® Green Assay: Capza1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: Capza1, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Capza1, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCID0021052
Assay Design:		Intron-spanning
Chromosome Location:		3:104844933-104864407question
Amplicon Length:		87
Splice Variants Targeted:		ENSMUST00000039476 ENSMUST00000025224 ENSMUST00000080036 ENSMUST00000167978 ENSMUST00000047485 ENSMUST00000105283 ENSMUST00000051377 ENSMUST00000010985 ENSMUST00000053355 ENSMUST00000047431 ENSMUST00000092840 ENSMUST00000138797 ENSMUST00000094028 ENSMUST00000020706 ENSMUST00000038141 ENSMUST00000130822 ENSMUST00000100716 ENSMUST00000048052 ENSMUST00000061111 ENSMUST00000043313 ENSMUST00000026254 ENSMUST00000176992 ENSMUST00000009219 ENSMUST00000161991 ENSMUST00000159951 ENSMUST00000034377 ENSMUS

Gene Information

Description Not Available

Gene Symbol:		Capza1
Gene Name:		Capping protein (actin filament) muscle Z-line, alpha 1
Aliases:		CAPZ, CAZ1, Cappa1
RefSeq:		NC_000069.6 NT_039240.8
Ensembl:		ENSMUSG00000070372
Entrez:		12340
Chromosome Mapping:		3 F2.2\|3 55.0 cM

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999900
y-intercept	35.740000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.