PrimePCR™ SYBR® Green Assay: Adra1d, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0021064
Assay Design:   Intron-spanning
Chromosome Location:   2:131546476-131561114question
Amplicon Length:   74
Splice Variants Targeted:   ENSMUST00000028771 ENSMUST00000089311 ENSMUST00000100439 ENSMUST00000046259 ENSMUST00000040182 ENSMUST00000050183 ENSMUST00000017836 ENSMUST00000113599 ENSMUST00000060837 ENSMUST00000091227 ENSMUST00000103184

Gene Information

Description Not Available

Gene Symbol:   Adra1d
Gene Name:   Adrenergic receptor, alpha 1d
Aliases:   Adra-1, Adra1, Adra1a, Gpcr8, Spr8, [a]1d, alpha1D-AR
RefSeq:   NC_000068.7 NT_039207.8
Ensembl:   ENSMUSG00000027335
Entrez:   11550
Chromosome Mapping:   2 F1|2 74.9 cM

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.810000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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