PrimePCR™ SYBR® Green Assay: Bcmo1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qMmuCIP0033785


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PrimePCR™ PreAmp for SYBR® Green Assay: Bcmo1, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Bcmo1, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCID0021268
Assay Design:		Intron-spanning
Chromosome Location:		8:117113497-117117427question
Amplicon Length:		97
Splice Variants Targeted:		ENSMUST00000034308 ENSMUST00000176860 ENSMUST00000167370

Gene Information

Vitamin A metabolism is important for vital processes such as vision embryonic development cell differentiation and membrane and skin protection. The protein encoded by this gene is a key enzyme in beta-carotene metabolism to vitamin A. It catalyzes the oxidative cleavage of betabeta-carotene into two retinal molecules. Two alternatively spliced variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2009]

Gene Symbol:		Bcmo1
Gene Name:		Beta-carotene 15,15'-monooxygenase
Aliases:		Bcdo, Bcdo1, CMO1, Cmoi, beta-CD, betaCMOOX
RefSeq:		NC_000074.6 NT_078575.7
Ensembl:		ENSMUSG00000031845
Entrez:		63857
Chromosome Mapping:		8 E1\|8

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	35.500000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.