PrimePCR™ SYBR® Green Assay: Gtf3a, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCIP0035042

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Gtf3a, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Gtf3a, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0022516
Assay Design:   Intron-spanning
Chromosome Location:   5:146948933-146951267question
Amplicon Length:   195
Splice Variants Targeted:   ENSMUST00000146511 ENSMUST00000132102

Gene Information

The product of this gene is a zinc finger protein with nine Cis[2]-His[2] zinc finger domains. It functions as an RNA polymerase III transcription factor to induce transcription of the 5S rRNA genes. The protein binds to a 50 bp internal promoter in the 5S genes called the internal control region (ICR) and nucleates formation of a stable preinitiation complex. This complex recruits the TFIIIC and TFIIIB transcription factors and RNA polymerase III to form the complete transcription complex. The protein is thought to be translated using a non-AUG translation initiation site in mammals based on sequence analysis protein homology and the size of the purified protein. [provided by RefSeq Jul 2008]

Gene Symbol:   Gtf3a
Gene Name:   General transcription factor III A
Aliases:   2010015D03Rik, 2610111I01Rik, 5330403M05Rik
RefSeq:   NC_000071.6 NT_039324.8
Ensembl:   ENSMUSG00000016503
Entrez:   66596
Chromosome Mapping:   5 G2|5

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