PrimePCR™ SYBR® Green Assay: Gdf11, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: Gdf11, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Gdf11, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0024392
Assay Design:   Intron-spanning
Chromosome Location:   10:128886497-128891319question
Amplicon Length:   92
Splice Variants Targeted:   ENSMUST00000078547 ENSMUST00000048432 ENSMUST00000050149 ENSMUST00000037991 ENSMUST00000150569 ENSMUST00000127648 ENSMUST00000026408 ENSMUST00000005255 ENSMUST00000108527 ENSMUST00000088987 ENSMUST00000102491 ENSMUST00000097816 ENSMUST00000168157 ENSMUST00000040222 ENSMUST00000092170 ENSMUST00000055322 ENSMUST00000061328 ENSMUST00000102810 ENSMUST00000049618

Gene Information

Description Not Available

Gene Symbol:   Gdf11
Gene Name:   Growth differentiation factor 11
Aliases:   BMP-11, Bmp11
RefSeq:   NC_000076.6 NT_039500.8
Ensembl:   ENSMUSG00000025352
Entrez:   14561
Chromosome Mapping:   10 D3|10

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.510000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download