PrimePCR™ SYBR® Green Assay: Sult2a4, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qMmuCIP0043570


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PrimePCR™ PreAmp for SYBR® Green Assay: Sult2a4, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Sult2a4, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCID0041354
Assay Design:		Intron-spanning
Chromosome Location:		7:13931713-13988214question
Amplicon Length:		169
Splice Variants Targeted:		ENSMUST00000108525 ENSMUST00000108522 ENSMUST00000108520 ENSMUST00000165167 ENSMUST00000098809

Gene Information

This is one of seven sulfotransferase family 2A genes in a chromosome 7 A1 cluster. [provided by RefSeq May 2010]

Gene Symbol:		Sult2a4
Gene Name:		Sulfotransferase family 2A, dehydroepiandrosterone (DHEA)-preferring, member 4
Aliases:		EG434121, Gm5584
RefSeq:		NC_000073.6 NT_187034.1
Ensembl:		ENSMUSG00000074377
Entrez:		434121
Chromosome Mapping:		7 A1\|7

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999100
y-intercept	40.170000
Efficiency	86

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.