PrimePCR™ SYBR® Green Assay: Flrt3, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qMmuCIP0043653


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PrimePCR™ PreAmp for SYBR® Green Assay: Flrt3, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Flrt3, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCID0041437
Assay Design:		Intron-spanning
Chromosome Location:		2:140651143-140663304question
Amplicon Length:		120
Splice Variants Targeted:		ENSMUST00000159674 ENSMUST00000022438 ENSMUST00000065587 ENSMUST00000094787 ENSMUST00000168667 ENSMUST00000021800 ENSMUST00000021527 ENSMUST00000119092 ENSMUST00000094491 ENSMUST00000024748 ENSMUST00000109155 ENSMUST00000063710 ENSMUST00000042561 ENSMUST00000108433 ENSMUST00000169547 ENSMUST00000099301 ENSMUST00000047793

Gene Information

Description Not Available

Gene Symbol:		Flrt3
Gene Name:		Fibronectin leucine rich transmembrane protein 3
Aliases:		Not Available
RefSeq:		Not Available
Ensembl:		ENSMUSG00000079021
Chromosome Mapping:		Not Available

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	36.480000
Efficiency	94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.