PrimePCR™ SYBR® Green Assay: Hist1h2bd, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: Hist1h2bd, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Hist1h2bd, Rat
Reaction: 4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qRnoCED0002518
Assay Design:		exonic
Chromosome Location:		17:58632378-58632506question
Amplicon Length:		99
Splice Variants Targeted:		ENSRNOT00000017005 ENSRNOT00000028779 ENSRNOT00000030960 ENSRNOT00000047596 ENSRNOT00000000718 ENSRNOT00000024872 ENSRNOT00000009737 ENSRNOT00000008211 ENSRNOT00000012644 ENSRNOT00000046129 ENSRNOT00000010784 ENSRNOT00000066190 ENSRNOT00000041187 ENSRNOT00000022630 ENSRNOT00000072195 ENSRNOT00000030198 ENSRNOT00000075811 ENSRNOT00000072293 ENSRNOT00000071688 ENSRNOT00000041423 ENSRNOT00000048465 ENSRNOT00000026032 ENSRNOT00000050005

Gene Information

Description Not Available

Gene Symbol:		Hist1h2bd
Gene Name:		Histone cluster 1, H2bd
Aliases:		Not Available
RefSeq:		Not Available
Ensembl:		ENSRNOG00000050927
Entrez:		361247
Chromosome Mapping:		17q11

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.999200
y-intercept	36.050000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.