PrimePCR™ SYBR® Green Assay: Cacna1d, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: Cacna1d, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Cacna1d, Rat
Reaction: 4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qRnoCED0002707
Assay Design:		exonic
Chromosome Location:		16:6077329-6077995question
Amplicon Length:		81
Splice Variants Targeted:		ENSRNOT00000051371 ENSRNOT00000040759 ENSRNOT00000048459 ENSRNOT00000049531 ENSRNOT00000043180 ENSRNOT00000045459 ENSRNOT00000017975 ENSRNOT00000047737 ENSRNOT00000027263

Gene Information

has high voltage L-type calcium channel activity that is sensitive to dihydropyridines [RGD, Feb 2006]

Gene Symbol:		Cacna1d
Gene Name:		Voltage-dependent L-type calcium channel subunit alpha-1D
Aliases:		Not Available
RefSeq:		Not Available
Ensembl:		ENSRNOG00000013147
Entrez:		29716
Chromosome Mapping:		16p16

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.999300
y-intercept	36.690000
Efficiency	96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.