PrimePCR™ SYBR® Green Assay: Hist2h2ac, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Hist2h2ac, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Hist2h2ac, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0003121
Assay Design:   exonic
Chromosome Location:   2:217846053-217846177question
Amplicon Length:   95
Splice Variants Targeted:   ENSRNOT00000075630 ENSRNOT00000072835 ENSRNOT00000046811 ENSRNOT00000007371 ENSRNOT00000051033 ENSRNOT00000003914 ENSRNOT00000018021 ENSRNOT00000074399 ENSRNOT00000066367 ENSRNOT00000050526 ENSRNOT00000022965 ENSRNOT00000013790 ENSRNOT00000059328 ENSRNOT00000074307 ENSRNOT00000074677 ENSRNOT00000045512 ENSRNOT00000074717 ENSRNOT00000073997 ENSRNOT00000047696 ENSRNOT00000073287 ENSRNOT00000044446 ENSRNOT00000051917 ENSRNOT00000044268 ENSRNOT00000059715 ENSRNOT00000041937 ENSRNOT00000042768

Gene Information

Description Not Available

Gene Symbol:   Hist2h2ac
Gene Name:   Histone H2A
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000030736
Entrez:   499675
Chromosome Mapping:   2q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.390000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download