PrimePCR™ SYBR® Green Assay: RT1-CE3, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: RT1-CE3, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RT1-CE3, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0003807
Assay Design:   exonic
Chromosome Location:   20:7008751-7008850question
Amplicon Length:   70
Splice Variants Targeted:   ENSRNOT00000048535 ENSRNOT00000047439 ENSRNOT00000049540 ENSRNOT00000004646 ENSRNOT00000046414 ENSRNOT00000015778 ENSRNOT00000061114 ENSRNOT00000047527 ENSRNOT00000075244 ENSRNOT00000061027 ENSRNOT00000071840 ENSRNOT00000050099 ENSRNOT00000049310 ENSRNOT00000041590 ENSRNOT00000031595 ENSRNOT00000007112 ENSRNOT00000050374 ENSRNOT00000036278 ENSRNOT00000010641

Gene Information

Description Not Available

Gene Symbol:   RT1-CE3
Gene Name:   RT1 class I, locus CE3 precursor
Aliases:   Not Available
RefSeq:   NM_001008841
Ensembl:   ENSRNOG00000031607
Entrez:   414793
Chromosome Mapping:   20p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.995800
y-intercept 36.990000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download