PrimePCR™ SYBR® Green Assay: Dnm1l, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Dnm1l, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Dnm1l, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0004536
Assay Design:   exonic
Chromosome Location:   11:91924672-91924775question
Amplicon Length:   74
Splice Variants Targeted:   ENSRNOT00000003572 ENSRNOT00000002478 ENSRNOT00000002479 ENSRNOT00000002482 ENSRNOT00000002477 ENSRNOT00000002485 ENSRNOT00000037094 ENSRNOT00000034762

Gene Information

human homolog is a GTPase that is necessary for peroxisome division [RGD, Feb 2006]

Gene Symbol:   Dnm1l
Gene Name:   Dynamin-1-like protein
Aliases:   Not Available
RefSeq:   NM_053655
Ensembl:   ENSRNOG00000001813
Entrez:   114114
Chromosome Mapping:   11q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.840000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download