PrimePCR™ SYBR® Green Assay: Taar7b, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: Taar7b, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Taar7b, Rat
Reaction: 4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qRnoCED0004640
Assay Design:		exonic
Chromosome Location:		1:23993812-23993944question
Amplicon Length:		103
Splice Variants Targeted:		ENSRNOT00000021545 ENSRNOT00000051416 ENSRNOT00000050763 ENSRNOT00000021559 ENSRNOT00000043436 ENSRNOT00000044271 ENSRNOT00000046379

Gene Information

neurotransmitter/neuromodulator of trace amines; responsible for psychiatric disorders, depression and migraine [RGD, Feb 2006]

Gene Symbol:		Taar7b
Gene Name:		Trace amine-associated receptor 7b
Aliases:		Not Available
RefSeq:		NM_175586
Ensembl:		ENSRNOG00000046302
Entrez:		294126
Chromosome Mapping:		1p12

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.999600
y-intercept	37.130000
Efficiency	102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.