PrimePCR™ SYBR® Green Assay: Dhps, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $183.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Dhps, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Dhps, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0006998
Assay Design:   exonic
Chromosome Location:   19:37161001-37161110question
Amplicon Length:   80
Splice Variants Targeted:   ENSRNOT00000005656

Gene Information

human homolog catalyzes the first step in the post-translational modification of a lysine residue in the precursor eukaryotic initiation factor 5A (eIF5A) to the amino acid hypusine [RGD, Feb 2006]

Gene Symbol:   Dhps
Gene Name:   Deoxyhypusine synthase
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000004219
Entrez:   288923
Chromosome Mapping:   19q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.420000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download