PrimePCR™ SYBR® Green Assay: RGD1564698, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: RGD1564698, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: RGD1564698, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0012819
Assay Design:   exonic
Chromosome Location:   10:19591897-19592045question
Amplicon Length:   119
Splice Variants Targeted:   ENSRNOT00000026597 ENSRNOT00000026914 ENSRNOT00000033907 ENSRNOT00000059426 ENSRNOT00000011633 ENSRNOT00000001292 ENSRNOT00000065157 ENSRNOT00000032917 ENSRNOT00000057435 ENSRNOT00000027763 ENSRNOT00000018782 ENSRNOT00000073428 ENSRNOT00000005953 ENSRNOT00000055950 ENSRNOT00000037890 ENSRNOT00000067532 ENSRNOT00000003295 ENSRNOT00000055410 ENSRNOT00000045306 ENSRNOT00000043925 ENSRNOT00000040278 ENSRNOT00000042029 ENSRNOT00000043250 ENSRNOT00000047113

Gene Information

Description Not Available

Gene Symbol:   RGD1564698
Gene Name:   Protein RGD1564698
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000030106
Entrez:   497882
Chromosome Mapping:   10q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 36.270000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download