PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000049841, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000049841, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000049841, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0020227
Assay Design:   exonic
Chromosome Location:   20:4570609-4570710question
Amplicon Length:   72
Splice Variants Targeted:   ENSRNOT00000048858 ENSRNOT00000000990 ENSRNOT00000052311 ENSRNOT00000030174 ENSRNOT00000040634 ENSRNOT00000051480 ENSRNOT00000041500 ENSRNOT00000039155 ENSRNOT00000042866 ENSRNOT00000050246 ENSRNOT00000074123 ENSRNOT00000071931 ENSRNOT00000061415 ENSRNOT00000048535 ENSRNOT00000061395 ENSRNOT00000036147 ENSRNOT00000050159 ENSRNOT00000042882 ENSRNOT00000048036 ENSRNOT00000001035 ENSRNOT00000001036 ENSRNOT00000047439 ENSRNOT00000070903 ENSRNOT00000061299 ENSRNOT00000041502 ENSRNOT00000048076 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000049841
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000049841
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 36.930000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download