PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000045643, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $188.00
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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000045643, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000045643, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0020902
Assay Design:   exonic
Chromosome Location:   16:69744795-69744928question
Amplicon Length:   104
Splice Variants Targeted:   ENSRNOT00000072406 ENSRNOT00000059247 ENSRNOT00000035049 ENSRNOT00000039250 ENSRNOT00000032631 ENSRNOT00000073167 ENSRNOT00000035638 ENSRNOT00000011239 ENSRNOT00000030319 ENSRNOT00000037416 ENSRNOT00000014849 ENSRNOT00000048888 ENSRNOT00000050117 ENSRNOT00000057412 ENSRNOT00000042570 ENSRNOT00000047001 ENSRNOT00000048860 ENSRNOT00000049689

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000045643
Gene Name:   Uncharacterized protein
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000045643
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.340000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download