PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000048777, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000048777, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000048777, Rat
Reaction: 4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qRnoCED0020969
Assay Design:		exonic
Chromosome Location:		2:184521694-184521798question
Amplicon Length:		75
Splice Variants Targeted:		ENSRNOT00000071873 ENSRNOT00000072012 ENSRNOT00000072688 ENSRNOT00000074316 ENSRNOT00000049456 ENSRNOT00000071140 ENSRNOT00000071352 ENSRNOT00000075615 ENSRNOT00000072079 ENSRNOT00000075488 ENSRNOT00000073190 ENSRNOT00000072157 ENSRNOT00000074064 ENSRNOT00000073232 ENSRNOT00000072415 ENSRNOT00000071457 ENSRNOT00000072358 ENSRNOT00000071087 ENSRNOT00000075660 ENSRNOT00000071813 ENSRNOT00000075737 ENSRNOT00000074044 ENSRNOT00000072650 ENSRNOT00000071379 ENSRNOT00000075688 ENSRNOT00000071244 ENSRNO

Gene Information

Description Not Available

Gene Symbol:		No symbol available for ENSRNOG00000048777
Gene Name:		Not Available
Aliases:		Not Available
RefSeq:		Not Available
Ensembl:		ENSRNOG00000048777
Entrez:		0
Chromosome Mapping:		Not Available

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.998500
y-intercept	36.340000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.