PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000050384, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000050384, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000050384, Rat
Reaction: 4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qRnoCED0021119
Assay Design:		exonic
Chromosome Location:		3:173687461-173687563question
Amplicon Length:		73
Splice Variants Targeted:		ENSRNOT00000030857 ENSRNOT00000033772 ENSRNOT00000042498 ENSRNOT00000031481 ENSRNOT00000070851 ENSRNOT00000073416 ENSRNOT00000008101 ENSRNOT00000008047 ENSRNOT00000075257 ENSRNOT00000075429 ENSRNOT00000042110 ENSRNOT00000063989 ENSRNOT00000075012 ENSRNOT00000075542 ENSRNOT00000072043 ENSRNOT00000049457 ENSRNOT00000074655 ENSRNOT00000072350 ENSRNOT00000073650 ENSRNOT00000043131 ENSRNOT00000066393 ENSRNOT00000075091 ENSRNOT00000049165 ENSRNOT00000068215

Gene Information

Description Not Available

Gene Symbol:		No symbol available for ENSRNOG00000050384
Gene Name:		Not Available
Aliases:		Not Available
RefSeq:		Not Available
Ensembl:		ENSRNOG00000050384
Entrez:		0
Chromosome Mapping:		Not Available

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.998700
y-intercept	36.590000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.