PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000029898, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000029898, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000029898, Rat
Reaction: 4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qRnoCED0021168
Assay Design:		exonic
Chromosome Location:		3:44171546-44171682question
Amplicon Length:		107
Splice Variants Targeted:		ENSRNOT00000012153 ENSRNOT00000002700 ENSRNOT00000075580 ENSRNOT00000004416 ENSRNOT00000022054 ENSRNOT00000040517 ENSRNOT00000072743 ENSRNOT00000015152 ENSRNOT00000009687 ENSRNOT00000043210 ENSRNOT00000029055 ENSRNOT00000005817 ENSRNOT00000035567 ENSRNOT00000028875 ENSRNOT00000057122 ENSRNOT00000032387 ENSRNOT00000025221

Gene Information

Description Not Available

Gene Symbol:		No symbol available for ENSRNOG00000029898
Gene Name:		Not Available
Aliases:		Not Available
RefSeq:		Not Available
Ensembl:		ENSRNOG00000029898
Entrez:		0
Chromosome Mapping:		Not Available

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.999600
y-intercept	35.770000
Efficiency	104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.