PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000046417, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000046417, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000046417, Rat
Reaction: 4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qRnoCED0021174
Assay Design:		exonic
Chromosome Location:		15:34141316-34141449question
Amplicon Length:		104
Splice Variants Targeted:		ENSRNOT00000072362 ENSRNOT00000075194 ENSRNOT00000073984 ENSRNOT00000071631 ENSRNOT00000071740 ENSRNOT00000073175 ENSRNOT00000064158 ENSRNOT00000072382 ENSRNOT00000071598 ENSRNOT00000073855

Gene Information

Description Not Available

Gene Symbol:		No symbol available for ENSRNOG00000046417
Gene Name:		Protein LOC100361487
Aliases:		Not Available
RefSeq:		Not Available
Ensembl:		ENSRNOG00000046417
Entrez:		0
Chromosome Mapping:		Not Available

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.997800
y-intercept	37.170000
Efficiency	88

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.