PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000048977, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000048977, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000048977, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0021179
Assay Design:   exonic
Chromosome Location:   1:80877489-80877592question
Amplicon Length:   74
Splice Variants Targeted:   ENSRNOT00000011956 ENSRNOT00000066363 ENSRNOT00000036178 ENSRNOT00000071396 ENSRNOT00000009762 ENSRNOT00000007112 ENSRNOT00000028855 ENSRNOT00000071194 ENSRNOT00000011365 ENSRNOT00000049961 ENSRNOT00000031074 ENSRNOT00000064128 ENSRNOT00000010070 ENSRNOT00000071316 ENSRNOT00000058230 ENSRNOT00000071154 ENSRNOT00000071041 ENSRNOT00000026107 ENSRNOT00000071814 ENSRNOT00000030893 ENSRNOT00000044029

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000048977
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000048977
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.340000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download