PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000047033, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $179.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0021315
Assay Design:   exonic
Chromosome Location:   19:40087155-40087299question
Amplicon Length:   115
Splice Variants Targeted:   ENSRNOT00000044037 ENSRNOT00000046676 ENSRNOT00000042781 ENSRNOT00000042150 ENSRNOT00000041115 ENSRNOT00000049865 ENSRNOT00000043494 ENSRNOT00000048218 ENSRNOT00000048506 ENSRNOT00000075616 ENSRNOT00000049208 ENSRNOT00000047660 ENSRNOT00000018858

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000047033
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000047033
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 36.270000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download