PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000049501, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000049501, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000049501, Rat
Reaction: 4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qRnoCED0021409
Assay Design:		exonic
Chromosome Location:		4:167881975-167882081question
Amplicon Length:		77
Splice Variants Targeted:		ENSRNOT00000058388 ENSRNOT00000047754 ENSRNOT00000030077 ENSRNOT00000072170 ENSRNOT00000001553 ENSRNOT00000066360 ENSRNOT00000058437 ENSRNOT00000074454 ENSRNOT00000074759 ENSRNOT00000048979 ENSRNOT00000058389 ENSRNOT00000071985 ENSRNOT00000040075 ENSRNOT00000028553 ENSRNOT00000065206 ENSRNOT00000033858 ENSRNOT00000026405 ENSRNOT00000049162 ENSRNOT00000071736 ENSRNOT00000031103 ENSRNOT00000074010 ENSRNOT00000074206 ENSRNOT00000073076 ENSRNOT00000075709 ENSRNOT00000072325 ENSRNOT00000058282 ENSRNO

Gene Information

Description Not Available

Gene Symbol:		No symbol available for ENSRNOG00000049501
Gene Name:		Not Available
Aliases:		Not Available
RefSeq:		Not Available
Ensembl:		ENSRNOG00000049501
Entrez:		0
Chromosome Mapping:		Not Available

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.999700
y-intercept	35.460000
Efficiency	106

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.