PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000048084, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000048084, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000048084, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0021798
Assay Design:   exonic
Chromosome Location:   20:1221973-1222121question
Amplicon Length:   119
Splice Variants Targeted:   ENSRNOT00000075301 ENSRNOT00000074751 ENSRNOT00000029778 ENSRNOT00000047450 ENSRNOT00000065895 ENSRNOT00000064798 ENSRNOT00000073543 ENSRNOT00000072457 ENSRNOT00000049250 ENSRNOT00000052186 ENSRNOT00000040957 ENSRNOT00000074181 ENSRNOT00000072222 ENSRNOT00000073732 ENSRNOT00000071933 ENSRNOT00000075267 ENSRNOT00000074388 ENSRNOT00000072544 ENSRNOT00000075252 ENSRNOT00000052052 ENSRNOT00000041189 ENSRNOT00000044130 ENSRNOT00000045794

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000048084
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000048084
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.840000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download