PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000050389, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000050389, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000050389, Rat
Reaction: 4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qRnoCED0021878
Assay Design:		exonic
Chromosome Location:		20:421685-421820question
Amplicon Length:		106
Splice Variants Targeted:		ENSRNOT00000075114 ENSRNOT00000048277 ENSRNOT00000044420 ENSRNOT00000051646 ENSRNOT00000047483 ENSRNOT00000046628 ENSRNOT00000040820 ENSRNOT00000049521 ENSRNOT00000041635 ENSRNOT00000048708 ENSRNOT00000047478

Gene Information

Description Not Available

Gene Symbol:		No symbol available for ENSRNOG00000050389
Gene Name:		Olfactory receptor Olr1675
Aliases:		Not Available
RefSeq:		Not Available
Ensembl:		ENSRNOG00000050389
Entrez:		0
Chromosome Mapping:		Not Available

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.998900
y-intercept	35.820000
Efficiency	102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.