PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000045700, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000045700, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000045700, Rat
Reaction: 4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qRnoCED0022044
Assay Design:		exonic
Chromosome Location:		12:22616925-22617071question
Amplicon Length:		117
Splice Variants Targeted:		ENSRNOT00000052327 ENSRNOT00000072303 ENSRNOT00000074970 ENSRNOT00000073627 ENSRNOT00000064718 ENSRNOT00000073947 ENSRNOT00000074034 ENSRNOT00000074057 ENSRNOT00000075640 ENSRNOT00000071130 ENSRNOT00000071430 ENSRNOT00000072097 ENSRNOT00000074863 ENSRNOT00000039797 ENSRNOT00000075641 ENSRNOT00000046100 ENSRNOT00000072653 ENSRNOT00000003540 ENSRNOT00000022144 ENSRNOT00000017571 ENSRNOT00000057161 ENSRNOT00000065865

Gene Information

Description Not Available

Gene Symbol:		No symbol available for ENSRNOG00000045700
Gene Name:		Not Available
Aliases:		Not Available
RefSeq:		Not Available
Ensembl:		ENSRNOG00000045700
Entrez:		0
Chromosome Mapping:		Not Available

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	35.670000
Efficiency	101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.