PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000039187, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000039187, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000039187, Rat
Reaction: 4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qRnoCED0022066
Assay Design:		exonic
Chromosome Location:		16:10448329-10448431question
Amplicon Length:		73
Splice Variants Targeted:		ENSRNOT00000066957 ENSRNOT00000059965 ENSRNOT00000060746 ENSRNOT00000064412 ENSRNOT00000071517 ENSRNOT00000046081 ENSRNOT00000029573 ENSRNOT00000075372 ENSRNOT00000072045 ENSRNOT00000031932 ENSRNOT00000036987 ENSRNOT00000075511 ENSRNOT00000010527

Gene Information

Description Not Available

Gene Symbol:		No symbol available for ENSRNOG00000039187
Gene Name:		Not Available
Aliases:		Not Available
RefSeq:		Not Available
Ensembl:		ENSRNOG00000039187
Entrez:		0
Chromosome Mapping:		Not Available

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.999900
y-intercept	36.410000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.