PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000045975, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000045975, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000045975, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0022142
Assay Design:   exonic
Chromosome Location:   9:41975972-41976087question
Amplicon Length:   86
Splice Variants Targeted:   ENSRNOT00000031915 ENSRNOT00000001265 ENSRNOT00000013161 ENSRNOT00000056321 ENSRNOT00000046791 ENSRNOT00000049138 ENSRNOT00000058595 ENSRNOT00000048420 ENSRNOT00000050803 ENSRNOT00000071995 ENSRNOT00000050291 ENSRNOT00000042619 ENSRNOT00000051772 ENSRNOT00000049571 ENSRNOT00000048356 ENSRNOT00000049363 ENSRNOT00000048115 ENSRNOT00000051901 ENSRNOT00000040249 ENSRNOT00000040493 ENSRNOT00000048373 ENSRNOT00000045446 ENSRNOT00000047936

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000045975
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000045975
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 35.660000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download