PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000021905, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000021905, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000021905, Rat
Reaction: 4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qRnoCED0022509
Assay Design:		exonic
Chromosome Location:		1:83574149-83574270question
Amplicon Length:		92
Splice Variants Targeted:		ENSRNOT00000009426 ENSRNOT00000046984 ENSRNOT00000046053 ENSRNOT00000030194 ENSRNOT00000048281 ENSRNOT00000058640 ENSRNOT00000047993 ENSRNOT00000050161 ENSRNOT00000040376 ENSRNOT00000022114 ENSRNOT00000040905 ENSRNOT00000031497 ENSRNOT00000072831 ENSRNOT00000045504 ENSRNOT00000057873 ENSRNOT00000044787 ENSRNOT00000046626 ENSRNOT00000070854 ENSRNOT00000075275 ENSRNOT00000049438 ENSRNOT00000047765 ENSRNOT00000047553

Gene Information

Description Not Available

Gene Symbol:		No symbol available for ENSRNOG00000021905
Gene Name:		Not Available
Aliases:		Not Available
RefSeq:		Not Available
Ensembl:		ENSRNOG00000021905
Entrez:		0
Chromosome Mapping:		Not Available

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.999600
y-intercept	35.880000
Efficiency	101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.