PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000050796, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000050796, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000050796, Rat
Reaction: 4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qRnoCED0022758
Assay Design:		exonic
Chromosome Location:		17:5950695-5950788question
Amplicon Length:		64
Splice Variants Targeted:		ENSRNOT00000072151 ENSRNOT00000072930 ENSRNOT00000037766 ENSRNOT00000074514 ENSRNOT00000030857 ENSRNOT00000033772 ENSRNOT00000042498 ENSRNOT00000047433 ENSRNOT00000043099 ENSRNOT00000034545 ENSRNOT00000037708 ENSRNOT00000042638 ENSRNOT00000061526 ENSRNOT00000073416 ENSRNOT00000038951 ENSRNOT00000048576 ENSRNOT00000008763 ENSRNOT00000008101 ENSRNOT00000008047 ENSRNOT00000075257 ENSRNOT00000075429 ENSRNOT00000042110 ENSRNOT00000063989 ENSRNOT00000051082 ENSRNOT00000072350 ENSRNOT00000043955 ENSRNO

Gene Information

Description Not Available

Gene Symbol:		No symbol available for ENSRNOG00000050796
Gene Name:		Not Available
Aliases:		Not Available
RefSeq:		Not Available
Ensembl:		ENSRNOG00000050796
Entrez:		0
Chromosome Mapping:		Not Available

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.999100
y-intercept	36.880000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.