PrimePCR™ SYBR® Green Assay: No symbol available for ENSRNOG00000021490, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: No symbol available for ENSRNOG00000021490, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: No symbol available for ENSRNOG00000021490, Rat
Reaction: 4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qRnoCED0022783
Assay Design:		exonic
Chromosome Location:		1:70469762-70469911question
Amplicon Length:		120
Splice Variants Targeted:		ENSRNOT00000020919 ENSRNOT00000073222 ENSRNOT00000074792 ENSRNOT00000074699 ENSRNOT00000042693 ENSRNOT00000073754 ENSRNOT00000007398 ENSRNOT00000037611 ENSRNOT00000034630 ENSRNOT00000074428 ENSRNOT00000073410 ENSRNOT00000073032 ENSRNOT00000072232 ENSRNOT00000073973 ENSRNOT00000048588 ENSRNOT00000050241 ENSRNOT00000033032 ENSRNOT00000050133 ENSRNOT00000048913 ENSRNOT00000048404 ENSRNOT00000044270 ENSRNOT00000049921 ENSRNOT00000051735

Gene Information

Description Not Available

Gene Symbol:		No symbol available for ENSRNOG00000021490
Gene Name:		Not Available
Aliases:		Not Available
RefSeq:		Not Available
Ensembl:		ENSRNOG00000021490
Entrez:		0
Chromosome Mapping:		Not Available

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.998400
y-intercept	36.720000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.