PrimePCR™ SYBR® Green Assay: Rpl31, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Rpl31, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Rpl31, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0051129
Assay Design:   Exonic
Chromosome Location:   9:45775075-45777022question
Amplicon Length:   85
Splice Variants Targeted:   ENSRNOT00000010838 ENSRNOT00000033733 ENSRNOT00000047997 ENSRNOT00000006851 ENSRNOT00000043590 ENSRNOT00000041674 ENSRNOT00000045401 ENSRNOT00000049211 ENSRNOT00000002177 ENSRNOT00000047517 ENSRNOT00000045604 ENSRNOT00000043610 ENSRNOT00000046725 ENSRNOT00000060519 ENSRNOT00000042248 ENSRNOT00000041763 ENSRNOT00000015408 ENSRNOT00000047850 ENSRNOT00000013596 ENSRNOT00000042885 ENSRNOT00000047507

Gene Information

ribosomal protein subunit [RGD, Feb 2006]

Gene Symbol:   Rpl31
Gene Name:   60S ribosomal protein L31
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000013508
Entrez:   64298
Chromosome Mapping:   9q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 37.230000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download