PrimePCR™ SYBR® Green Assay: Hist1h2ah, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: Hist1h2ah, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Hist1h2ah, Rat
Reaction: 4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qRnoCED0052017
Assay Design:		Exonic
Chromosome Location:		17:45487020-45487174question
Amplicon Length:		125
Splice Variants Targeted:		ENSRNOT00000030497 ENSRNOT00000004298 ENSRNOT00000018349 ENSRNOT00000033917 ENSRNOT00000066367 ENSRNOT00000050526 ENSRNOT00000022965 ENSRNOT00000013790 ENSRNOT00000059328 ENSRNOT00000074307 ENSRNOT00000074677 ENSRNOT00000045512 ENSRNOT00000074717 ENSRNOT00000073997 ENSRNOT00000047696 ENSRNOT00000073287 ENSRNOT00000044446 ENSRNOT00000051917 ENSRNOT00000044268 ENSRNOT00000027188

Gene Information

Description Not Available

Gene Symbol:		Hist1h2ah
Gene Name:		Histone H2A type 1-E
Aliases:		Not Available
RefSeq:		Not Available
Ensembl:		ENSRNOG00000049649
Entrez:		502125
Chromosome Mapping:		17p11

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.999600
y-intercept	37.720000
Efficiency	93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.