PrimePCR™ SYBR® Green Assay: Phkg2, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: Phkg2, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Phkg2, Rat
Reaction: 4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qRnoCED0052636
Assay Design:		Exonic
Chromosome Location:		1:206027545-206027726question
Amplicon Length:		152
Splice Variants Targeted:		ENSRNOT00000066508 ENSRNOT00000035009 ENSRNOT00000019577 ENSRNOT00000071069 ENSRNOT00000018637 ENSRNOT00000070815 ENSRNOT00000017746 ENSRNOT00000007954 ENSRNOT00000056416 ENSRNOT00000003750 ENSRNOT00000056415 ENSRNOT00000024932 ENSRNOT00000021286 ENSRNOT00000025419

Gene Information

phosphorylase kinase catalytic subunit isoform; catalyzes the phosphorylation and activation of glycogen phosphorylase [RGD, Feb 2006]

Gene Symbol:		Phkg2
Gene Name:		Phosphorylase b kinase gamma catalytic chain, testis/liver isoform
Aliases:		Not Available
RefSeq:		Not Available
Ensembl:		ENSRNOG00000018725
Entrez:		140671
Chromosome Mapping:		1q36

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.997700
y-intercept	41.570000
Efficiency	94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.