PrimePCR™ SYBR® Green Assay: F2rl3, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0052972
Assay Design:   Exonic
Chromosome Location:   16:18688916-18689061question
Amplicon Length:   116
Splice Variants Targeted:   ENSRNOT00000072664

Gene Information

G-protein-coupled receptor activated by thrombin and trypsin; mediates gastrointestinal motility [RGD, Feb 2006]

Gene Symbol:   F2rl3
Gene Name:   Proteinase-activated receptor 4
Aliases:   Not Available
RefSeq:   NM_053808
Ensembl:   ENSRNOG00000048186
Entrez:   116498
Chromosome Mapping:   16

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 37.330000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download