PrimePCR™ SYBR® Green Assay: Pidd, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Pidd, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Pidd, Rat
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qRnoCED0055831
Assay Design:   Exonic
Chromosome Location:   1:221340044-221340147question
Amplicon Length:   74
Splice Variants Targeted:   ENSRNOT00000060929 ENSRNOT00000049285 ENSRNOT00000020305 ENSRNOT00000035491 ENSRNOT00000003840 ENSRNOT00000006191 ENSRNOT00000024895 ENSRNOT00000058725 ENSRNOT00000061236 ENSRNOT00000059639 ENSRNOT00000072587 ENSRNOT00000044293 ENSRNOT00000010516 ENSRNOT00000030090 ENSRNOT00000032077 ENSRNOT00000007069 ENSRNOT00000055087 ENSRNOT00000055093 ENSRNOT00000045430 ENSRNOT00000038156 ENSRNOT00000028409 ENSRNOT00000018000 ENSRNOT00000035001 ENSRNOT00000010070 ENSRNOT00000000474 ENSRNOT00000072774 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   Pidd
Gene Name:   Protein Lrdd
Aliases:   Not Available
RefSeq:   NM_001106318
Ensembl:   ENSRNOG00000018710
Entrez:   293625
Chromosome Mapping:   1q41

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.670000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download