PrimePCR™ SYBR® Green Assay: Aadat, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: Aadat, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Aadat, Rat
Reaction: 4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qRnoCID0004832
Assay Design:		intron_spanning
Chromosome Location:		16:32695007-32698105question
Amplicon Length:		118
Splice Variants Targeted:		ENSRNOT00000015974

Gene Information

endogenous modulator of glutamatergic neurotransmission with kynurenine aminotransferase (KAT) activity [RGD, Feb 2006]

Gene Symbol:		Aadat
Gene Name:		Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial
Aliases:		Not Available
RefSeq:		NM_017193
Ensembl:		ENSRNOG00000011861
Entrez:		29416
Chromosome Mapping:		16p12

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.998800
y-intercept	35.240000
Efficiency	106

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.